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Rio et al., Science, 227: 11 (1985) [PMID: 2981116] Pear et al., Proc. Natl. Acad. Sci. USA, 90: 11 [PMID: 7690960] CABRI:DSMZ_MUTZ Accession_number ACC 635 Cell_line_name 293T Brief_description confirmed as human by species PCR highly transfectable derivative of the human primary embryonal kidney cell line 293 (ACC 305) carrying a plasmid containing the temperature sensitive mutant of SV-40 large T-antigen (tsA1609); cells were described to express SV-40 large T at 33°C, but not at 40°C; classified as risk group Description human embryonal kidney highly transfectable derivative of the human primary embryonal kidney cell line 293 (ACC 305) carrying a plasmid containing the temperature sensitive mutant of SV-40 large T-antigen (tsA1609); cells were described to express SV-40 large T at 33°C, but not at 40°C; classified as risk group 1 according to the German Central Commission for Biological Safety (ZKBS) confirmed as human by species PCR Depositor Dr. S. Nagel, DSMZ, Braunschweig, Germany Morphology fibroblastoid cells growing adherently as monolayer Culture_conditions 90% Dulbecco´s MEM + 10% FBS seed out at ca. 2-3 x 106 cells/80 cm 2 ; split confluent culture 1:4 - 1:5 every 2-3 days (without using trypsin/EDTA, cells can be detached by tapping the flask) at 37C with 5% CO2 cell harvest of ca. 40 x 106 cells/175 cm 2 Viruses PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV - Properties cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, EpCAM -, neurofilament -, vimentin + Release_conditions - Hazard - Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule Doubling_time ca. 24-30 hours Mycoplasma negative in microbiological culture, PCR assays Fingerprint same DNA profile as 293 (ACC 305) by fluorescent nonaplex PCR of short tandem repeat markers Cytogenetics human flat-moded near-triploid karyotype; 61-73<3n>XXX, add(1)(p32), add(1)(q44)x1-2, der(4;10)(q10;q10), del(8)(p12), t(9;9)(p24;q21), der(13)t(6;13)(q21;q32), der(14)t(13;14)(q32;q32), del(17)(p11), der(22)t(17;22)(q21;q22) Bibliographic_reference DuBridge et al., Mol. Cell Biol., 7: 11 (1987) [PMID: 3031469 ]

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